HPTN Bibliographic Record

Gamiel JL, Tobian AA, Laeyendecker OB, Reynolds SJ, Ashley-Morrow R, Swerwadda D, Gray RH, and Quinn TC. Improved performance of enzyme linked immunosorbent assays and the effect of human immunodeficiency virus co-infection on the serologic detection of herpes simplex virus type-2 in Rakai Uganda. Clin Vaccine Immunol. 2008, 15: 888-890. PMC:394849
Ugandan subjects (820) were tested by Focus HerpeSelect enzyme-linked immunosorbent assay (ELISA), Kalon herpes simplex virus type 2 ELISA, and BioKit rapid test, and the results were compared to those of Western blotting. Higher-than-standard-index cutoff values gave optimal sensitivity and specificity. Kalon ELISA was the optimal assay when an index value of 1.5 was used (sensitivity, 91.7%; specificity, 92.4%).