Lynch BA, Patel EU, Courtney CR, Nanfack AJ, Bimela J, Wang X, Eid I, Quinn TC, Laeyendecker O, Nyambi PN, Duerr R, Redd AD. Short Communication: False Recent Ratio of the Limiting-Antigen Avidity Assay and Viral Load Testing Algorithm Among Cameroonians with Long-Term HIV Infection. AIDS Res Hum Retroviruses. 2017, 33: 1114-1116. PMC5665419
Current serological assays that are used for cross-sectional HIV incidence estimation have been shown to misclassify individuals with chronic infection. Limited information exists on the performance of cross-sectional incidence assays in Central Africa. HIV-positive individuals from Cameroon who were infected for at least 1 or 2 years were evaluated to determine the false recent ratio (FRR) of a two-assay algorithm, which includes the Limiting Antigen Avidity (LAg-Avidity) assay (normalized optical density units, ODn <1.5) and HIV viral load (>1000 copies/ml). The subject-level FRR was 5.3% (95% confidence interval [CI], 2.1-10.5) for individuals infected for >/=1 year and 3.9% (95% CI, 0.8-11.0) for individuals infected for >/=2 years. These data suggest that the LAg-Avidity plus viral load incidence algorithm may overestimate HIV incidence rates in Central Africa.